Manufacturing method for silica encapsulated single-enzyme nanoparticles and single-enzyme nanoparticles manufactured by means of method

摘要

A method for manufacturing single enzyme nanoparticles through silica encapsulation according to the present disclosure does not include a surface functionalization process and a polymerization process during the synthesis, and reaction conditions are mild. Thus, the method is appropriate for a large scale production.

主权项

1. Silica encapsulated single-enzyme nanoparticles comprising a single enzyme and a silica network surrounding the single enzyme and having pores, wherein no covalent bond is formed between the single enzyme and the silica network, and the silica encapsulated single-enzyme nanoparticles have a Michaelis-Menten constant (Km) value with a margin of error not greater than 20% as compared to that of free enzymes, and wherein the silica network has a thickness of 0.5-2.0 nm.