Anti-NPC2 monoclonal antibodies and a method of detecting fatty liver tissue, cancer cells or cancer tissue by using them

摘要

The present invention is related to anti-NPC2 monoclonal antibodies, which against NPC2 or glycosylated-NPC2; and is related to a method of detecting fatty liver tissues, cancer cells or cancer tissues by evaluating the expression level of NPC2 or glycosylated-NPC2 in the cells or tissues.

主权项

1. An isolated and biologically pure NPC2 (Niemann-Pick C2) monoclonal antibody for detecting fatty liver, wherein:
the monoclonal antibody specifically binds an N-terminal epitope contained in the amino acid sequence of NPC2 protein consisting of SEQ ID NO: 3; the monoclonal antibody can specifically bind to the NPC2 protein whether the NPC2 protein is glycosylated or not; the monoclonal antibody comprises an IgG2a heavy chain and kappic light chain; and the OD490 value of the monoclonal antibody binding to the N-terminal epitope is about 1.6 to 1.7.