| 摘要 |
PURPOSE:To obtain the subject enzyme with a simple operation in high productivity with making possible of a high-density cultivation of strain by controlling concentration of methanol with successively adding methanol using concentration of dissolved oxygen in a medium as an index. CONSTITUTION:Methanol is successively added using a methanol-utilizing microorganism and concentration of dissolved oxygen in a medium is used as an index to afford the aimed enzyme by a cultivation method. Besides, a methanol- utilizing yeast: candida boidinii is preferably used as said microorganism. Furthermore, as a culturing condition, e.g. an aerobic cultivation in stirring with ventilation at 28 deg.C for 24-72hr is preferable. |
