VERFAHREN ZUR HOHEN BELADUNG VON VESIKELN MIT BIOPOLYMEREN SUBSTANZEN

摘要

A method for high loading of vesicles with biopolymeric substances functional in human biochemical or physiological systems derived from any source, said substances preferably are enzymes, proenzymes, cofactors, submicron particles such as virions, growth factors, cytokines, ribosomes, hepatitis B surface antigen, oligo- and polynucleotides, antibodies and/or antigens said vesicles formed by amphiphatic substances comprising the steps of a) mixing lipids suitable for forming lipid vesicles in water-immiscible organic solvents, alternatively, dried lipids or lipid mixture can be used in any form (powder, granular, etc.) directly, b) removing of the solvent in presence of a solid support c) taking up the product of step b) into a solution of the substances to be encapsulated in a physiologically compatible solution d) adding an organic solvent having solubilizing or disposing properties, as well as e) drying the fraction obtained in step d) under conditions retaining the function of the substances to be encapsulated.