发明名称 |
METHODS AND COMPOSITIONS FOR INTERACTION TRAP ASSAYS |
摘要 |
The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 10<7> members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids. |
申请公布号 |
WO0188197(A2) |
申请公布日期 |
2001.11.22 |
申请号 |
WO2001US15718 |
申请日期 |
2001.05.16 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
JOUNG, J., KEITH;MILLER, JEFFREY;PABO, CARL, O. |
分类号 |
C12N15/10;C12Q1/68;G01N33/50;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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