发明名称 |
IMPROVEMENTS TO RIBOSOME DISPLAY |
摘要 |
The use in a ribosome display system for a selection of a specific binding pair member (e.g. antibody molecule) able to bind a complementary specific binding pair member (e.g. antigen) of encapsidating specific binding member/ribosome complexes ina viral coat, optionally in combination with incorporation of an Midvariant RNA template and optionally one or more other improvements selected from : glycine-serine tether, protein disulphide isomerase, protein disulphide isomerase in combination with oxidised and reduced glutathione at a ratio of between 1:1 and 10:1, addition of oxidised and reduced glutathione at a ratio of between 1:1 and 10:1 after 30 minutes of <i>in vitro</i> translation; blocking with heparin during selection.
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申请公布号 |
WO0175097(A3) |
申请公布日期 |
2002.01.03 |
申请号 |
WO2001GB01319 |
申请日期 |
2001.03.26 |
申请人 |
CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED;OSBOURN, JANE;HOLET, THOR |
发明人 |
OSBOURN, JANE;HOLET, THOR |
分类号 |
C12N15/09;C07K16/00;C07K16/28;C07K16/44;C12N7/00;C12N7/04;C12N15/10;C12Q1/68;C40B40/02;C40B50/06;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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