Recombinant yeast transformant and process for preparing immunoglobulin Fc fragment employing the same

摘要

The present invention relates to a transformant prepared by introducing an expression vector comprising a polynucleotide encoding for a human immunoglobulin Fc fragment into Pichia sp. yeast, a method for producing an immunoglobulin Fc fragment comprising culturing the transformant, and recovering the immunoglobulin Fc fragment from the culture, and an immunoglobulin Fc fragment, prepared by the above method for use as a drug carrier. The transformant is suggested as a solution to the problems associated with the use of E. coli or animal cells as hosts for producing immunoglobulin Fc fragments useful as drug carriers, so that it can find various applications in the effective and economical production of drugs.

主权项

1. A transformant, modified by introducing an expression vector comprising a polynucleotide encoding a human immunoglobulin Fc fragment into Pichia sp. Yeast, wherein the expression vector is pPIC9K-IgG4Fc having the cleavage map of FIG. 1, or pPIC9K-mPSCFc having the cleavage map of FIG. 2.