FURIN-KNOCKDOWN AND GM-CSF-AUGMENTED (FANG) CANCER VACCINE

摘要

The present invention includes compositions and methods for cancer treatment. More specifically the present invention describes an autologous cancer vaccine genetically modified for Furin knockdown and GM-CSF expression. The vaccine described herein attenuates the immunosuppressive activity of TGF-3 through the use of bi-functional shRNAs to knock down the expression of furin in cancer cells, and to augment tumor antigen expression, presentation, and processing through expression of the GM-CSF transgene. WO 2011/079077 PCT/US2010/061344 GMCSF Expression In Pre & Post FANG Plasmid Transfected Autologous Tumor Cells (Day 4) 2000 2000 ""-""F A NG -00 1 ~FANG-002 1500 FANG-005 e. F ANG-06 1000 - FANG-007 FANG-00 500 ----F A N G -0 103 j1g. ic TGF.1 Expression by FANG-004 (NSCLC) pre and post transfection/post irradiated cells -2000 0 r DayO Dayl Day2 Day3 Day4 Day7 Day10 Dayl4 jiN. 20A