Therapeutic and diagnostic strategies

摘要

The present invention encompasses the finding that microRNAs (miRNAs) regulate certain key proteins involved in DNA repair. In some embodiments, a miRNA suppresses levels and/or activity of one or more DNA repair proteins. In some such embodiments, such suppression renders cells hypersensitive to certain DNA damage agents (e.g., γ-irradiation and genotoxic drugs, among others). The present invention provides various reagents and methods associated with these findings including, among other things, strategies for treating cell proliferative disorders, certain diagnostic systems, etc.

主权项

1. A method comprising steps of:
administering to an individual who is suffering from or susceptible to a cell proliferative disorder a composition comprising an miR-24 agent in an amount sufficient to inhibit cell proliferation in combination with a DNA damage agent and/or cell proliferation disorder treatment method that produces DNA double-strand breaks, isolating cells from the individual administered the miR-24 agent and preparing Wright-stained metaphase chromosome spreads from the isolated cells or staining the isolated cells from the individual with SYBR Green and performing single cell gel electrophoresis on the isolated cells, thereby measuring DNA damage and detecting DNA damage repair to be reduced in the isolated cells, as compared to control cells, thereby identifying the individual administered the miR-24 agent to possess reduced DNA damage repair, further comprising administering to the individual so identified to possess reduced DNA damage repair the miR-24 agent and γ-irradiation or a genotoxic drug.