System and method for quantifying fragile X mental retardiation 1 protein in tissue and blood samples

摘要

A system and method for the detection and quantification of fragile X mental retardation protein (FMRP) in human tissue and blood samples. The system includes several high avidity monoclonal antibodies that may be provided on Xmap microspheres to capture FMRP from a tissue or blood specimen. The resulting complex is reacted with a polyclonal anti-FMRP rabbit antibody and then mixed with an anti-rabbit IgG antibody conjugated to phycoerythrin. Fluorescence emitted from the resulting complex is a function of the amount of FMRP present in the specimen.

主权项

1. An assay system for detecting a fragile X mental retardation protein in a human tissue sample, comprising:
a monoclonal mouse antibody that binds to SEQ. ID NO. 3; a polyclonal antibody that binds to a second, different epitope of the fragile X mental retardation protein; and a third antibody that binds to said polyclonal antibody and is conjugated to a fluorescent compound.