METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES

摘要

The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

主权项

1. A non-naturally occurring microbial organism comprising a genetic alteration selected from the group consisting of:
(1) attenuation of cydA or cydB, and one or more of a genetic alteration selected from the group consisting of:
(a) a genetic alteration that increases expression of a protein encoded by pntAB;(b) attenuation of the protein encoded by pykF;(c) attenuation of the protein encoded by sucCD;(d) attenuation of the protein encoded by yciA;(e) a genetic alteration that increases expression of a protein encoded by ackA and a protein encoded by pta;(f) a genetic alteration that increases expression of a protein encoded by cyoB;(g) attenuation of the protein encoded by pykA;(h) attenuation of the protein encoded by arcA;(i) attenuation of the protein encoded by crr;(j) attenuation of the protein encoded by clpA; and(k) attenuation of the protein encoded by menC; and (2) a genetic alteration selected from the group consisting of
(a) attenuation of the proteins encoded by sucCD and yciA;(b) attenuation of the proteins encoded by sucCD and yciA, and having a genetic alteration that increases expression of a protein encoded by pntAB;(c) attenuation of the proteins encoded by sucCD and yciA, and having a genetic alteration that increases expression of a protein encoded by cyoB;(d) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by cyoB;(e) attenuation of the proteins encoded by sucCD, yciA and menC and having a genetic alteration that increases expression of a protein encoded by cyoB;(f) attenuation of the proteins encoded by sucCD, yciA, cydA or cydB and menC and having a genetic alteration that increases expression of an protein encoded by cyoB;(g) attenuation of the proteins encoded by sucCD and cydA or cydB;(h) attenuation of the proteins encoded by sucCD and cydA or cydB and pykF;(i) attenuation of the proteins encoded by sucCD and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;(j) attenuation of the proteins encoded by sucCD and cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;(k) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB;(l) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and pykF;(m) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;(n) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;(o) attenuation of the proteins encoded by sucCD, yciA, and cydA or cydB and menC;(p) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and menC, and having a genetic alteration that increases expression of a protein encoded by pntAB;(q) attenuation of the proteins encoded by sucCD and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;(r) attenuation of the protein encoded by clpA;(s) attenuation of the protein encoded by menC;(t) attenuation of the protein encoded by menC and cydA or cydB;(u) attenuation of the protein encoded by pykF and/or pykA;(v) having a genetic alteration that increases expression of a protein encoded by pntAB;(w) attenuation of cydA or cydB and sucCD, arcA and crr, and having a genetic alteration that increases expression of a protein encoded by ackA and pta;(x) attenuation of cydA or cydB and arcA;(y) attenuation of cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;(z) attenuation of cydA or cydB and pykF; and(aa) attenuation of cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB.