METHOD OF PRODUCING ERYTHROCYTES WITHOUT FEEDER CELLS

摘要

Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perforate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrate erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.

主权项

1. A method of producing erythrocytes, comprising expanding a population of hematopoietic cells in a medium in the absence of feeder cells and in the contact with one or more factors, wherein a plurality of hematopoietic cells within said population of hematopoietic cells differentiate into erythrocytes during said expanding; and isolating said erythrocytes from said medium,
wherein said factors comprise SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01 to about 500 ng/mL, and Epo at a concentration of about 0.1 to about 10 IU/mL, said medium does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4 (HoxB4), or methylcellulose, and wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium.