发明名称 |
Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery |
摘要 |
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c). |
申请公布号 |
US9522218(B2) |
申请公布日期 |
2016.12.20 |
申请号 |
US200812681682 |
申请日期 |
2008.10.10 |
申请人 |
INSERM (Institut National de la Santé et de la Recherche Médicale);Université Paris 7—Denis Diderot |
发明人 |
Le Visage Catherine;Letourneur Didier |
分类号 |
A61L27/20;C08J3/075;C08B37/00;C08B37/02;C08B37/04;C08B37/10;C08B37/12;C08J9/08;A61L27/52;A61L27/56;A61L27/38;A61L27/50;C12N5/00;C08B37/08;C08J9/28 |
主分类号 |
A61L27/20 |
代理机构 |
McAndrews, Held & Malloy, Ltd. |
代理人 |
McAndrews, Held & Malloy, Ltd. |
主权项 |
1. A method for preparing a porous scaffold which consists of the following steps:
a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide selected from the group consisting of dextran, agar, pullulan, inulin, heparin, fucoidan, and mixtures thereof; an amount of a covalent cross-linking agent selected from the group consisting of trisodium trimetaphosphate (STMP), phosphorus oxychloride (POCl3), epichlorohydrin, formaldehydes, and glutaraldehydes; and an amount of a porogen agent selected from the group consisting of ammonium carbonate, ammonium bicarbonate, calcium carbonate, sodium carbonate, and sodium bicarbonate and mixtures thereof; b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide; c) submerging said hydrogel into an aqueous acidic solution to form pores; and d) washing the porous scaffold obtained at step c), wherein the porous scaffold is a hydrogel; the porogen agent is dissolved in step a); the weight ratio of the polysaccharide to the porogen agent is in the range from 6:1 to 1:1; and wherein the alkaline aqueous solution in a) does not include a surfactant. |
地址 |
Paris FR |