发明名称 |
METHODS AND COMPOSITIONS FOR IDENTIFYING MORPHOGEN ANALOGS |
摘要 |
Disclosed herein are methods and compositions for identifying morphogen analogs. The preferred methods and compositions relate to the discovery that morphogen upregulation of the mouse type X collagen promoter activity is mediated by a MEF-2 like sequence and required an adjacent AP-1 sequence. Certain methods rest on the use of test cells comprising DNA defining a morphogen-responsive transcription activating element operatively associated with a reporter gene. Other methods rest on the use of DNAs for measuring morphogen-inducible DNA-binding. In certain preferred embodiments, the methods and DNAs involve an osteogenic protein 1 (OP-1) responsive transcription activating element. Substances that mediate interaction with and/or activate the OP-1 responsive transcription activating element are considered herein likely to be useful for reproducing in vivo effects of morphogens such as OP-.
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申请公布号 |
WO9826047(A1) |
申请公布日期 |
1998.06.18 |
申请号 |
WO1997US23192 |
申请日期 |
1997.12.11 |
申请人 |
CREATIVE BIOMOLECULES, INC. |
发明人 |
SAMPATH, KUBER, T. |
分类号 |
C12N15/09;A61K31/00;A61K35/12;A61K38/00;A61K38/27;A61K45/00;A61P1/00;A61P1/04;A61P1/16;A61P9/00;A61P9/10;A61P19/00;A61P19/08;A61P25/28;A61P29/00;A61P43/00;C07K14/51;C12N5/00;C12N5/10;C12P21/02;C12Q1/68;C12R1/91;(IPC1-7):C12N5/16;C12N15/12;C12N15/85;A61K38/18 |
主分类号 |
C12N15/09 |
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