发明名称 |
PRODUCTION OF VACCINES USING TRANSGENIC PLANTS |
摘要 |
Plants infected with potato virus Y (PVY) were inoculated with infectious PVX RNA encoding PVX coat protein (CP) and modified chimeric NDV/BYMV CP. Antigen-coated plate indirect ELISA and immunoelectron microscopy of virus purified from infected plants showed that progeny virions contained from <1% to as much as 25% chimeric CP. The method can be used to produce and purify large amounts of NDV vaccine. Transencapsidated PVY virions expressing NDV were purified and used to induce anti-NDV antibodies in mice. Further, transgenic tobacco plants expressing HIV/BYMV CP were inoculated with BYMV. Progeny virions from transgenic plants contained transgenic HIV/BYMV CP. |
申请公布号 |
WO0200169(A3) |
申请公布日期 |
2002.07.18 |
申请号 |
WO2001US20272 |
申请日期 |
2001.06.26 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE |
发明人 |
HAMMOND, ROSEMARIE;ZHAO, YAN;HAMMOND, JOHN |
分类号 |
C07K14/08;C07K14/125;C07K14/16;C12N15/82 |
主分类号 |
C07K14/08 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|