| 主权项 |
1. An isolated nucleic acid molecule consisting of:
a) a sequence from nucleotide position 118 up to and including nucleotide position 3945 of the nucleic acid sequence shown in SEQ ID NO 1 or SEQ ID NO 3; b) a sequence which codes for a protein whose amino acid sequence has at least 95% identity with the sequence from amino acid position 40 up to and including amino acid position 1315 of SEQ ID NO 2, wherein said protein has the same number of amino acids as the protein with the sequence from the amino acid at position 40 up to and including to the amino acid at position 1315 of SEQ ID NO 2; c) a sequence that has at least 95% identity with the sequence of a), where said sequence has the same number of nucleotides as the sequence of a), and encodes the protein of b); d) a sequence that, as the result of the degeneracy of the genetic code, deviates from the sequence of the nucleic acid molecules of a), b), or c); or e) a sequence that is complementary to the full-length sequence of one of the nucleic acid molecules of a), b), c), or d), where the complementary sequence has the same number of nucleotides as the nucleic acid molecules of a), b), c), or d);wherein said nucleic acid molecule codes for a truncated protein lacking the C-terminal domain and having enzymatic activity of an alternan sucrase, and wherein said nucleic acid molecule has attached to its 3′ end a nucleic acid triplet of TAA, TAG or TGA. |
