METHOD OF INCREASING OR INHIBITING GENE EXPRESSION BY MAKING USE OF PROMOTERS OF GLUTAMATE DEHYDROGENASE, {BETA}-N-HEXOSOANIMIDASE GENE AND GAMMA-ACTIN PROMOTERS AND USE OF SUCH PROMOTERS FOR PREPARING ANTIBIOTICS

摘要

The invention relates to promoters of the genes glutamate dehydrogenase, beta-acetylhexosaminidase and gamma-actin and their use in systems of expression, secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate dehydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) gamma-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) gamma-actin of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. These promoters can also be used for blocking the genic expression through anti-sense construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and/or proteins inherent to the same.