Biocoated Piezoelectric Biosensor Platform for Point-of-care Diagnostic Use

摘要

Abstract Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezoelectrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table monolayers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biotinylated antibody for capture and detection of analytics in biologic fluid samples. Although the processes described herein can be performed on any type of piezoelectric material in any number of configurations, some embodiments are directed to a biosensor with the foregoing biocoating onto a particular acoustic plate mode biosensor and where the interdigitated transducers (IDTs) are present on the opposite side of the crystal's biocoated film. WO 20131033049 PCTIJS2OII21052583 Figure 11, Panel B 0 ---- 0-- $----N - -_ 0 ------------/--- / / Iv V