Nucleic acid encoding a polypeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid

摘要

A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.

主权项

1. A DNA consisting of a nucleotide sequence encoding a polypeptide selected from the group consisting of:
(a) a polypeptide consisting of an amino acid sequence that is identical to the amino acid sequence of SEQ ID NO: 1 except for a deletion, a substitution, an insertion and/or an addition of 1 to 20 amino acids in SEQ ID NO: 1, wherein said polypeptide acts on 1-benzyl-3-pyrrolidinone in the presence of an amino group donor to generate (S)-1-benzyl-3-aminopyrrolidine with optical purity of 93% enantiomeric excess or more; and (b) a polypeptide consisting of an amino acid sequence that is identical to the amino acid sequence of SEQ ID NO: 1 except for a deletion, a substitution, an insertion and/or an addition of 1 to 20 amino acids in SEQ ID NO: 1, wherein said polypeptide acts on 1-benzyl-3-pyrrolidinone in the presence of an amino group donor to generate (S)-1-benzyl-3-aminopyrrolidine with optical purity of 93% enantiomeric excess or more; and wherein said polypeptide (i) exhibits activity for (S)-1-phenethylamine, benzylamine, and 2-butylamine as amino group donors and does not substantially exhibit activity for β-alanine and 4-aminobutyric acid, (ii) exhibits activity for pyruvic acid and activity for glyoxalic acid as amino group receptors; and/or (iii) retains 10% or more of residual activity after 2 hours of treatment with a solution having a concentration of 80% v/v of 1-propanol, 2-propanol, or acetone compared to the enzymatic activity prior to the treatment.