COMPOSITIONS AND METHODS FOR PREPARING OLIGONUCLEOTIDE SOLUTIONS

摘要

The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.

主权项

1. A method of generating a pool of oligonucleotides comprising:
(a) providing a substrate and at least first and second different oligonucleotides linked to said substrate through first and second cleavable linkers, respectively; and (b) cleaving said first and second linkers, thereby releasing said first and second oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said first and second oligonucleotides.