| 摘要 |
<p>A method for assaying chiro-inositol which comprises treating a liquid specimen with: 1) a dehydrogenase capable of undergoing a reversible reaction with at least chiro-inositol as the substrate by using a coenzyme selected from among NAD(P)s and another one selected from among thioNAD(P)s, in the presence of: 2) A1, and 3) B1, to thereby effect a cycling reaction represented by reaction scheme (I), wherein the product means a compound formed by eliminating two or four hydrogen atoms from chiroinositol; A1 represents one selected from among NAD(P)s and thioNAD(P)s; A2 represents the reduction product of A1; B1 represents a reduced NAD(P) when A1 is a thioNAD(P), or a reduced thioNAD(P) when A1 is an NAD(P); and B2 represents the oxidization product of B1, and then determining the amount of A2 or B1 changed by this reaction; and compositions for assaying chiro-inositol. Thus, chiro-inositol can be accurately, conveniently and economically assayed at a high sensitivity.</p> |
