Preparation of composition comprising purified hyaluronidase

摘要

Production of a preparation containing hyaluronidase (I) involves (1) comminuting mammalian (especially steer) testes to a slurry, (2) eluting proteins and enzymes from with acid, (3) fractionally precipitating the eluate with ammonium sulphate (AS) to form a (I)-containing precipitate fraction, (4) dialysing this fraction to remove AS, (5) purifying the dialysate by adsorption-desorption on hydrated alumina and (6) using the purified (I) to produce galenic formulations. The novel features are that AS precipitation is effected in two steps, firstly at 10-29 wt.% then at 15-65 wt.%; and that the purified fraction from (5) is subjected to ulltrafiltration at an exclusion limit of 5-30 kD before formulating in step (6).