发明名称 |
Methods for detecting LP-PLA2 activity and inhibition of LP-PLA2 activity |
摘要 |
This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor. |
申请公布号 |
US9465029(B2) |
申请公布日期 |
2016.10.11 |
申请号 |
US201514700053 |
申请日期 |
2015.04.29 |
申请人 |
Glaxo Group Limited |
发明人 |
Shou Yaping;Siu Yin Fai Amy;Walker George T. |
分类号 |
G01N33/573;C12Q1/44;G01N33/58 |
主分类号 |
G01N33/573 |
代理机构 |
|
代理人 |
Sauermelch Loretta J.;Gimmi Edward R. |
主权项 |
1. A colorimetric method of determining the percentage of inhibition of Lp-PLA2 activity, the method comprising:
preparing a solution comprising a substrate for Lp-PLA2 having a colorimetric detectable moiety, which substrate is 1-myristoyl-2-(4-nitrophenylsuccinyl)phosphatidylcholine; contacting a first blood sample that is taken from a human subject at a first time with the solution; colorimetrically detecting Lp-PLA2 activity from the first blood sample wherein the first blood sample is diluted less than 33 times; contacting a second blood sample that is taken from the subject at a second time one hour or more after the first time with the solution, wherein the second blood sample is taken after the subject has been administered a drug that inhibits Lp-PLA2; colorimetrically detecting Lp-PLA2 activity from the second blood sample wherein the second blood sample is diluted less than 33 times; and detecting greater than 30% inhibition of Lp-PLA2 activity between the first and second blood samples by comparing the activity of the Lp-PLA2 between the first and second blood samples, indicating that the subject has taken a drug that inhibits Lp-PLA2. |
地址 |
Middlesex GB |