发明名称 |
DRUG SCREEN TARGETING LIPOPROTEIN-MEMBRANE ANCHORAGE |
摘要 |
A drug screening assay for identifying compounds for their potential effects on the long chain fatty acid dependant intracellular membrane anchorage sites of lipoproteins. Membranes-anchoring-target are incubated in the presence of the compound to be tested and the proportion of the target that is released by the compound is detected and quantitated. The benchmark for this assay are the prenylated proteins such as the farnesylated and palmitoylated oncogenic ras trigger proteins which are displaced from their anchorage at the intracellular plasma membrane by derivatives of Farnesyl Thiosalicylic Acid (FTS). The assay is adaptable to flexibly assay a large variety of anchored targets using a wide range of labeling and detection techniques in test wells, tissue culture, and in animals as injected cells or transgenic, thereby directly addressing a wide range of pharmacologically relevant needs.
|
申请公布号 |
WO9838509(A1) |
申请公布日期 |
1998.09.03 |
申请号 |
WO1998US03669 |
申请日期 |
1998.02.26 |
申请人 |
THYREOS CORPORATION;HNAKLAI, RONI;PAZ, ARIELLA;KLOOG, YOEL |
发明人 |
HNAKLAI, RONI;PAZ, ARIELLA;KLOOG, YOEL |
分类号 |
G01N33/50;G01N33/15;G01N33/566;G01N33/567;G01N33/92;(IPC1-7):G01N33/48;G01N33/58;G01N33/574;C12Q1/42;C12Q1/48 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|