| 摘要 |
Determination of frame-shift mutations in coding target nucleic acids comprises: (i) providing a host cell comprising a double-stranded nucleic acid, which comprises a target nucleic acid and de-encode to a complementary nucleic acid encoding opposite strand, in which the opposite strand via a linker nucleic acid with a reporter gene in 3'-position is associated; (ii) effecting the expression of the opposite strand nucleic acid; and (iii) determining whether the host cell is an expression of the reporter gene, which indicates that the target nucleic acid has no frame-shift mutation-points. Determination of frame-shift mutations in coding target nucleic acids comprises: (i) providing a host cell comprising a double-stranded nucleic acid, which comprises a target nucleic acid and de-encode to a complementary nucleic acid encoding opposite strand, in which the opposite strand via a linker nucleic acid with a reporter gene in 3'-position is associated; (ii) effecting the expression of the opposite strand nucleic acid; and (iii) determining whether the host cell is an expression of the reporter gene, where expression of the reporter indicates that the target nucleic acid is no frame-shift mutation-points, the linker sequence comprises a translational coupler, the one stop codon in frame with the reading frame of the opposite strand nucleic acid comprises a start codon, and the reporter is in frame to the start codon. |
