Method for detection of high risk human papillomavirus

摘要

The invention provides compositions and methods for the differential detection of high risk forms of HPV from a urine sample provided by a patient. Specifically, the invention provides primers and probes that specifically recognize and bind sequences within the E1 gene of HPV. Detection of high risk forms of HPV identifies individuals at risk of developing or in the early stages of cervical carcinoma.

主权项

1. A method of detecting a high risk human papillomavirus (HPV) infection in a patient, comprising:
(a) obtaining a urine sample from a patient; and (b) detecting one or more sequences of the El gene of HPV in said urine sample by amplifying DNA in the urine sample using a forward primer having the sequence 5′-CAGGCAGAATTAGAGRCAGC (SEQ ID NO:41 or 99), wherein R is A or G, and detecting the amplification product; wherein detecting one or more sequences of the E1 gene of HPV indicates the presence of at least one high risk HPV in the patient.