发明名称 |
High performance liquid chromatography mobile phase. |
摘要 |
<p>There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing .005 to 1 M of a physiologically acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% V of a physiologically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA using reversed phase HPLC is also described.</p> |
申请公布号 |
EP0206792(A2) |
申请公布日期 |
1986.12.30 |
申请号 |
EP19860304799 |
申请日期 |
1986.06.23 |
申请人 |
MASSEY UNIVERSITY |
发明人 |
POLL, DICK JACOB;HARDING, DAVID ROGER KAY;HANCOCK, WILLIAM STEPHAN |
分类号 |
G01N30/34;B01D15/08;B01D15/42;C07K1/16;C07K1/20;(IPC1-7):C07K3/20 |
主分类号 |
G01N30/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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