发明名称 |
Use of multivalent inorganic cations in the electrochemical treatment of nucletic acid containing solutions |
摘要 |
PCT No. PCT/GB93/00147 Sec. 371 Date Nov. 18, 1994 Sec. 102(e) Date Nov. 18, 1994 PCT Filed Jan. 22, 1993 PCT Pub. No. WO93/15224 PCT Pub. Date Aug. 5, 1993A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process employs a promoter which is an inorganic multivalent cation such as magnesium ions to speed denaturation. The process may be used in the detection of nucleic acid by hybridrising with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.
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申请公布号 |
US5607832(A) |
申请公布日期 |
1997.03.04 |
申请号 |
US19940256784 |
申请日期 |
1994.11.18 |
申请人 |
SCIENTIFIC GENERICS LIMITED |
发明人 |
STANLEY, CHRISTOPHER J.;ARCHER, PATRICIA L. |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;G01N33/48 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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