MULTIDRUG ANALYSIS IN URINE BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

摘要

A fast and reliable method for the determination of multiple drugs and their metabolites belonging to different chemical and toxicological class from a biological sample is provided. The method involves mixing of biological sample with internal standards which does not require sample extraction or derivatization prior to analysis. Further the samples were analyzed by scheduled multiple reaction monitoring using liquid chromatography tandem mass spectrometer (LC-MS/MS).

主权项

1. A method of detection and/or quantification of multiple drugs and/or metabolites from a sample of body fluid comprising the steps:
(a) mixing the sample with internal standard, (b) hydrolyzing the drug metabolite in the sample by P-glucuronidase enzyme, (c) centrifugation of the mixture of step (b) and diluting the clear supernatant liquid with deionized water, and (d) analyzing said sample using liquid chromatography tandem mass spectrometer (LC-MS-MS) to determine the concentration of different drug metabolites;wherein, the method is devoid of solid and/or liquid phase extraction and/or derivatization.