FLUIDIC CHIP FOR FLOW CYTOMETRY AND METHODS OF USE

摘要

The present disclosure in some embodiments provides a flow cell sorting system, a focus detection method, as well as a fluidic chip. In one aspect, the fluidic chip comprises a cover sheet and one or more substrate layers, and one or more channels in each of the fluidic chips. In one aspect, the cells or other analytes in a sample are focused onto the same plane as they flow through the fluidic channel. In another aspect, the cells or other analytes on the same plane are irradiated with the same light intensity, for example, by a flat-top laser beam. This combination eliminates or reduces variation caused by cells at different positions receiving different intensities of the excitation light, and therefore improves accuracy of the flow cytometry analysis. In another aspect, a sorting apparatus is designed at the junction of the detection area and the outlet channel to separate the target cells.

主权项

1. A fluidic chip for analyzing a sample, comprising:
a fluidic channel comprising an inlet for adding a sample, and an outlet; a driving region and an optical detection region which are arranged sequentially in the direction of sample flow from the inlet to the outlet, wherein:
the driving region is an ultrasound driving region configured to provide an ultrasound to focus analytes in the sample onto substantially the same plane as the analytes flow through the fluidic channel in the ultrasound driving region, and the optical detection region is configured to provide a light to irradiate the analytes as they flow through the fluidic channel in the optical detection region after being focused onto substantially the same plane, such that the analytes on the same plane receive substantially the same intensity of light irradiation; orthe driving region is configured to provide a force to focus analytes in the sample onto substantially the same plane as the analytes flow through the fluidic channel in the driving region, and the optical detection region is configured to provide a flat-top light beam to irradiate the analytes as they flow through the fluidic channel in the optical detection region after being focused onto substantially the same plane, such that the analytes on the same plane receive substantially the same intensity of light irradiation; orthe driving region is an ultrasound driving region configured to provide an ultrasound to focus analytes in the sample onto substantially the same plane as the analytes flow through the fluidic channel in the ultrasound driving region, and the optical detection region is configured to provide a flat-top light beam to irradiate the analytes as they flow through the fluidic channel in the optical detection region after being focused onto substantially the same plane, such that the analytes on the same plane receive substantially the same intensity of light irradiation.