发明名称 |
METHODS FOR PURIFICATION OF MESSENGER RNA |
摘要 |
The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration. |
申请公布号 |
SG11201608725Y(A) |
申请公布日期 |
2016.11.29 |
申请号 |
SG11201608725Y |
申请日期 |
2015.04.24 |
申请人 |
SHIRE HUMAN GENETIC THERAPIES, INC. |
发明人 |
DEROSA, FRANK;DIAS, ANUSHA;KARVE, SHRIRANG;HEARTLEIN, MICHAEL |
分类号 |
C12N1/08;C07H21/00;C12N15/10 |
主分类号 |
C12N1/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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