发明名称 |
Method for Producing Myo-Inositol and Myo-Inositol Derivative |
摘要 |
To impart significantly improved myo-inositol producing capability, suitable for use in recombinant DNA techniques and synthetic biology methods, to a host microorganism that does not possess an endogenous myo-inositol biosynthesis pathway, such as Escherichia coli. Inositol monophosphatase activity is strengthened in a transformant obtained by introducing a myo-inositol biosynthesis pathway into a host microorganism that does not possess an endogenous myo-inositol biosynthesis pathway. |
申请公布号 |
US2016194667(A1) |
申请公布日期 |
2016.07.07 |
申请号 |
US201615076830 |
申请日期 |
2016.03.22 |
申请人 |
Asahi Kasei Chemicals Corporation |
发明人 |
Konishi Kazunobu;Imazu Shinichi;Sato Mayumi |
分类号 |
C12P7/02;C07H15/207;C12N9/16;C12N9/90 |
主分类号 |
C12P7/02 |
代理机构 |
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代理人 |
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主权项 |
1. A method of producing myo-inositol or a myo-inositol derivative, comprising:
(1) preparing a transformant of a microorganism that does not express endogenous inositol-1-phosphate synthase, the transformant containing at least an inositol-1-phosphate synthase-encoding exogenous gene introduced expressibly into the transformant, and the transformant having a gene recombination or mutation to induce overproduction of functional inositol monophosphatase or activation of inositol monophosphatase within the transformant; and (2) contacting the transformant with a carbon source that is converted to the compound of claim 21 by the transformant under conditions suited to growth and/or maintenance of the transformant. |
地址 |
Tokyo JP |