POLYNUCLEOTIDE ENCODING ACYL-COA SYNTHETASE HOMOLOG AND USE THEREOF

摘要

The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus Mortierella, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g., Mortierella alpina, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.

主权项

1. A polynucleotide, which is a cDNA, according to any one selected from the group consisting of (a) to (d) below:
(a) a polynucleotide comprising the nucleotide sequence shown by SEQ ID NO: 58; (b) a polynucleotide encoding a protein consisting of the amino acid sequence shown by SEQ ID NO: 57; (c) a polynucleotide encoding a protein consisting of an amino acid sequence wherein 1 to 10 amino acids are deleted, substituted, inserted and/or added in the amino acid sequence shown by SEQ ID NO: 57, and having an acyl-CoA synthetase activity or an activity of increasing the amount or changing the composition, of the fatty acids produced in a host cell when expressed in the host cell; and (d) a polynucleotide encoding a protein having an amino acid sequence having at least 95% identity to the amino acid sequence shown by SEQ ID NO: 57, and having an acyl-CoA synthetase activity or an activity of increasing the amount or changing the composition, of the fatty acids produced in a host cell when expressed in the host cell.