| 摘要 |
The invention is in the field of biotechnology and production of useful biomolecules. It relates to a method for culturing eukaryotic cells, more in particular adherent cells. The method is useful for the in vitro generation of tissue, for the production of biomolecules such as proteins and for use in regenerative medicine. The invention provides a bioreactor system comprising a membrane and a matrix suitable for cells to adhere to. The invention provides a bioreactor suitable for culturing adherent cells in a culture medium wherein said bioreactor comprises an internal chamber (11) and an external chamber (12) separated by a selective permeable membrane (22), the internal chamber comprising a three dimensional matrix (23) suitable for supporting the adherence of said cells, said matrix being surrounded by the selective permeable membrane, wherein the bioreactor comprises a feed medium inlet (31) and a feed medium outlet (32), both connected to the internal chamber and a spent medium inlet (41) and spent medium outlet (42), both connected to the external chamber.
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