| 摘要 |
<p>A method for identifying Mycobacterium tuberculosis and non-tuberculous mycobacteria is provided to conveniently and accurately differentiate Mycobacterial species by using each 8 signature nucleotide sequences capable of characterizing Mycobacterium tuberculosis group and non-tuberculous mycobacteria group. The method comprises the steps of: (a) amplifying a gene fragment including at least one base selected from the group consisting of bases located at 228th, 243th, 543th, 600th, 705th, and 718-720th from a 5'-terminal of a heat shock protein 65(HSP65) consisting of total 1623bp of Mycobacterial species using a primer specifically amplifying thereof; (b) analyzing the nucleotide sequence of the amplified gene fragment; and (c) comparing the bases above to identify non-tuberculous mycobacteria and Mycobacterium tuberculosis, where the non-tuberculous mycobacteria is 228th base of C, 243th base of C, 543th base of C, 600th base of C or T, 705th base of G or 718-720th bases of CAG, and the Mycobacterium tuberculosis is 228th base of A, 243th base of T, 543th base of T, 600th base of G, 705th base of C or 718-720th bases of GGA. The primer pair for producing PCR amplification product specific to the non-tuberculous mycobacteria is described as SEQ ID : NO. 1 and SEQ ID : NO.2. The differentiation kit for non-tuberculous mycobacteria and Mycobacterium tuberculosis comprises a primer pair described as SEQ ID : NOs. 1 to 4.</p> |
