| 摘要 |
The present invention relates to a transfection system for producing a genetically modified animal. With the transfection system according to the present invention, a viral vector can be used in order to effect direct genetic modification in an adult animal, such that not only is it possible to cell-specifically regulate inhibition of expression of a target gene by changing only the cell-specific promoter sequence of the vector and the shRNA sequence of the target gene but it is also possible to inhibit gene expression at a desired time by including a stop codon between two sequences recognised by a site-specific recombination enzyme. Consequently, it is expected that the transfection system according to the present invention can be easily used in studies into the roles of diverse genes, because the transfection system is able to cell-specifically inhibit the expression of a target gene at a desired time in various species of adult animals, when used. |
