DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES

摘要

A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.

主权项

1. A method comprising contacting one or more surfaces with a paper in the collection of evidence for a criminal investigation, depositing the paper into a vessel, performing a real-time polymerase chain reaction (rtPCR) within the vessel and detecting the level of fluorescence emitted from the vessel during rtPCR, wherein the level of fluorescence is detected by a charge-coupled device while the paper is in the vessel.