Eukaryotic host cell line in which an endogenous gene has been activated, and uses thereof

摘要

The invention relates to the a eukaryotic host cell line, obtained by the following process:
- inserting by homologous recombination a DNA construct comprising at least a DNA regulatory segment F which activates or modifies expression of a preselected gene in a predetermined eukaryotic host cell line when operatively linked thereto, a DNA targeting segment homologous to a region of the genome within or proximal to the preselected gene within the host cell line, and a selectable marker;
- chemical selection, and
- monitoring for production of the specific gene product of the newly activated gene in the eukaryotic host cell line.

<Paragraphs id="pa02" num="0002" xmlns:base="http://www.sipo.gov.cn/XMLSchema/base">The cell line is used for production of a specific gene product.