AKT-SPECIFIC CAPTURE AGENTS, COMPOSITIONS, AND METHODS OF USING AND MAKING

摘要

The present application provides stable peptide-based Akt capture agents and the use thereof as detection, diagnosis, and treatment agents. The application further provides novel methods of developing stable peptide-based capture agents, including Akt capture agents, using iterative on-bead in situ click chemistry.

主权项

1. A stable, synthetic triligand capture agent that specifically binds Akt, wherein the capture agent comprises an anchor ligand, a secondary ligand, and a tertiary ligand, and the capture agent binds to a non-ATP and non-peptide substrate binding site of Akt and wherein binding of said capture agent to the binding site allosterically inhibits Akt activity.