Method and system for selective isolation of target biological molecules in a general purpose system

摘要

Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques.

主权项

1. An apparatus for isolating biological molecules from a sample, comprising:
a substrate having a surface configured to adsorb a plurality of types of biological molecules, wherein the plurality of types of biological molecules comprises at least nucleic acids and proteins; a plurality of reservoirs, wherein each reservoir of the plurality of reservoirs houses a solution adapted to extract a specific type of biological molecule from the sample and to enhance adsorption of the specific type of biological molecule to the surface, and each solution housed in a respective reservoir of the plurality of reservoirs is adapted to extract and enhance adsorption of a different type of biological molecule from the other solutions housed in the other reservoirs; a valve coupled to the plurality of reservoirs via tubing; an injection port coupled to the valve and configured to receive the sample; a reaction mixing tube coupled to the valve and configured to receive the sample and a selected solution from one reservoir of the plurality of reservoirs; and a processor programmed to automatically select which of the solutions from the plurality of reservoirs should contact with the sample based at least in part on the type of biological molecule to be isolated, to control the valve to cause the selected solution and the sample to flow to the reaction mixing tube for mixing, and to control the valve to cause a mixture of the selected solution and the sample to be diverted from the reaction mixing tube to the substrate.