Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions for methods of treating tissue injury

摘要

The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

主权项

1. A method of producing a composition for treating tissue in need of repair comprising:
culturing fibroblast cells under hypoxic conditions on a microcarrier bead surface in a suitable growth medium, under 1-5% oxygen for at least 2 weeks, thereby producing multipotent stem cells, wherein the multipotent stem cells produce and secrete a composition that promotes repair and regeneration of damaged tissue when administered to the region of tissue in need of repair in a subject and collecting the growth medium after the 2 weeks, thereby producing the composition.