| 发明名称 |
Allele-specific amplification |
| 摘要 |
The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3′-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3′-terminal nucleotide. |
| 申请公布号 |
US9506111(B2) |
申请公布日期 |
2016.11.29 |
| 申请号 |
US201314052416 |
申请日期 |
2013.10.11 |
| 申请人 |
Roche Molecular Systems, Inc. |
发明人 |
Will Stephen G.;Tsan Alison;Newton Nicolas |
| 分类号 |
C07H21/02;C07H21/04;C12Q1/68 |
主分类号 |
C07H21/02 |
| 代理机构 |
|
代理人 |
Chang David J.;Savari M. Reza |
| 主权项 |
1. A kit for allele-specific amplification of a target sequence, which exists in the form of several variant sequences, comprising
(a) a first oligonucleotide, at least partially complementary to one or more variant of the target sequence; and (b) a second oligonucleotide, at least partially complementary to one or more variant of the target sequence and having a 3′-terminal nucleotide complementary to only one variant of the target sequence; wherein said second oligonucleotide is selected from a group consisting of SEQ ID NOs: 4, 5, 7, 8, 10, 11, and 18. |
| 地址 |
Pleasanton CA US |
