Glucose and xylose co-utilization in <i>E. coli </i>

摘要

The present invention provides host cells having improved sugar utilization or co-utilization, methods of producing host cells having improved sugar utilization or co-utilization, and methods of using host cells having improved sugar utilization or co-utilization. The present invention provides E. coli strains that co-utilize glucose and xylose in the presence of glucose and xylose, wherein the cell produces the product.

主权项

1. A method for producing an end-product from a biomass substrate comprising glucose and xylose, the method comprising:
providing an E. coli host cell, wherein the host cell has a phosphoenolpyruvate-dependent phosphotransferase transfer system negative (PTS−) phenotype and wherein the host cell has been modified to express:
(i) a first gene encoding a polypeptide having glucose transport activity, wherein the first gene is operably linked to a first heterologous promoter, and wherein the polypeptide having glucose transport activity comprises the amino acid sequence of SEQ ID NO:2; and(ii) a second gene encoding a polypeptide having xylose transport activity, wherein the second gene is operably linked to a second heterologous promoter, and wherein said second gene encodes a polypeptide comprising the sequence of SEQ ID NO:5;contacting the modified host cell with the biomass substrate; andculturing the modified host cell under suitable culture conditions, wherein said end-product is produced.