| 主权项 |
1. A method for producing an alkene from a 3-hydroxycarboxylate wherein a 3-hydroxycarboxylate of the following general formula I: wherein R1 and R3 are independently selected from hydrogen (—H), methyl (—CH3), ethyl (—CH2—CH3), isopropyl (—CH2(CH3)2), vinyl (—CH═CH2) and isopropenyl (—C(CH3)═CH2) and in which R2 and R4 are independently selected from hydrogen (—H) and methyl (—CH3), is, in a first step, enzymatically converted together with a co-substrate of the following formula II: wherein X is selected from the group consisting of O—PO3H2 monophosphate, O—PO2H—O—PO3H2 diphosphate, and O—SO3H sulfate, and wherein Y is selected from the group consisting of OH hydroxyl and O—PO3H2 monophosphate, and wherein Z is a nucleobase selected from the group consisting of adenine, guanine, thymine, cytosine, uracil and hypoxanthine, and wherein W is selected from the group consisting of hydrogen (—H) hydroxyl (OH), into a 3-hydroxycarboxyl-nucleotidylate of the following general formula III: wherein R1, R2, R3 and R4 have the same meaning as specified above in connection with formula I and wherein W, Y and Z have the same meaning as specified above in connection with formula II, and in that the thus produced 3-hydroxycarboxyl-nucleotidylate is subsequently enzymatically converted into an alkene of the following general formula IV: wherein R1, R2, R3 and R4 have the same meaning as specified above in connection with formula I. |
