Methods for 3-dimensional interferometric microscopy

摘要

A method includes sequentially acquiring phase-shifted images of an optical source in a plurality of predetermined positions in the z plane to form a plurality of image frame sequences; determining from the plurality of image frame sequences at least one phase-related characteristic associated with each predetermined position of the optical source in the z plane; and storing the at least one phase-related characteristic and the predetermined positions of the optical source in the z plane.

主权项

1. A method comprising:
interfering optical energy emitted from a fiducial optical emitter in at least two directions to produce N fiducial output beams along N legs, where N is greater than or equal to 2; sequentially moving the fiducial optical emitter in a plurality of predetermined positions along a z axis; acquiring images based on each of the N fiducial output beams as the fiducial optical emitter is moved along the plurality of predetermined positions along the z axis to form an image frame sequence for each of the N fiducial output beams; determining, at each of the N legs and from the plurality of image frame sequences, at least one characteristic associated with each predetermined position of the fiducial optical emitter along the z axis; and storing the at least one characteristic and the predetermined positions of the fiducial optical emitter along the z axis.