METHOD FOR GENERATING A CONTRAST IMAGE OF AN OBJECT STRUCTURE AND APPARATUSES RELATING THERETO

摘要

The invention relates to a contrast formation method, for generating a contrast image, including the steps: Illuminating an object by an illumination sequence based on one or more illumination sources; creating an illumination image of the object for each illumination in the illumination sequence; overlaying two respective illumination images which neighbour each other with respect to a first axis to form a first total axis image of the first axis; overlaying two respective illumination images which neighbour each other with respect to a second axis to form a second total axis image of the second axis; creating a first colour gradient image based on the first total axis image; creating a second colour gradient image based on the second total axis image; transforming the first colour gradient image and the second colour gradient image into a colour space; and generating a contrast image, based on the transformed first colour gradient image and the second colour gradient image into the colour space.

主权项

1. Contrast formation method for generating a contrast image, preferably from microscopic images, in particular for reading height profile information of an object structure from the contrast image, comprising the following steps:
illuminating (10) an object by means of an illumination sequence (11) based on one or more illumination sources (111); creating (20) an illumination image (21) of the object for each illumination (12) in the illumination sequence (11); overlaying (30) two respective illumination images (21) which neighbour each other with respect to a first axis (35) to form a first total axis image (31) of the first axis (35); overlaying (30) two respective illumination images (21) which neighbour each other with respect to a second axis (36) to form a second total axis image (32) of the second axis (36); creating (40) a first colour gradient image (41) based on the first total axis image (31); creating (40) a second colour gradient image (42) based on the second total axis image (32); transforming (50) the first colour gradient image (41) and the second colour gradient image (42) into a colour space (51); and generating (60) a contrast image (61), based on the transformed (50) first colour gradient image (41) and the second colour gradient image (42) into the colour space (51), such that four pieces of directional information (351, 352, 361, 362) of a height or phase profile of a structure of the object can be represented in colour-coded fashion.