SYSTEM, METHOD AND APPARATUS FOR ULTRA-RESOLVED ULTRA-WIDE FIELD-OF-VIEW MULTISPECTRAL AND HYPERSPECTRAL HOLOGRAPHIC MICROSCOPY

摘要

There is disclosed a novel system and method for achieving ultra-resolution, ultra-wide field-of-view multispectral and hyperspectral holographic microscopy and quantitative phase contrast microscopy. In an embodiment, the method comprises: providing a stationary illumination source; acquiring a plurality of low-resolution holograms of an image subject from different locations utilizing a subpixel sensor-scanning synthetic aperture mechanism whereby a detector scanning translationally, radially and/or rotationally; processing the acquired holograms utilizing a processing algorithm corresponding to the scanning motion of the detector used to acquire the holograms; and reconstructing a subpixel ultra-resolution image of the image subject based on the processed holograms; whereby, a desired synthetic aperture is achieved without loss of resolution. The multispectral and hyperspectral aspect is achieved in the novel system and method by use of different combination of illumination sources (i.e., LEDs, laser sources, broadband lamps, etc.) and wavelength selection mechanisms (i.e., bandpass spectral filters, acousto-optical and liquid crystal tunable filters, a dispersing element, etc.).

主权项

1. A method of performing holographic microscopy and quantitative phase contrast microscopy, comprising:
providing a stationary spectral illumination source with capability of central wavelength and spectral bandwidth selection for emission of light at desired wavelengths; acquiring with a detector a plurality of low-resolution holograms of an image subject from incremental locations utilizing a subpixel sensor-scanning synthetic aperture mechanism having one or more detector scanning patterns; processing the acquired holograms utilizing one or more processing algorithms corresponding to the one or more detector scanning patterns used to acquire the holograms; and reconstructing a subpixel ultra-resolution image of the image subject with an effective field-of-view (FOV) larger than the detector based on the plurality of processed low-resolution holograms; whereby, a desired synthetic aperture is achieved without loss of resolution.