发明名称 |
METHOD FOR SORTING OUT HOMOLOGOUS RECOMBINANT CELL |
摘要 |
<p>PURPOSE:To obtain a method for sorting out a homologous recombinant cell, applicable even to a gene unexpressed with an embryonic undifferentiated (ES) cell and having a high sorting out efficiency. CONSTITUTION:The objective method for sorting out a homologous recombinat cell is characterized by using a genome DNA comprising a neo gene having a PGK promoter on the upstream side thereof and an MVM pause signal and a blue script DNA inserted into a region between the neo gene and an MC1 promoter of a diphtherial toxin A fragment gene in a method for homologously recombining the genome DNA in which the neo gene and the diphtherial toxin A fragment gene having the MC1 promoter integrated therein in an embryonic undifferentiated (ES) cell and sorting out a cell containing a genome DNA having a deficient or modified gene therein with G418 and diphtherial toxin A.</p> |
申请公布号 |
JPH05328966(A) |
申请公布日期 |
1993.12.14 |
申请号 |
JP19920158592 |
申请日期 |
1992.05.26 |
申请人 |
RIKAGAKU KENKYUSHO |
发明人 |
YAGI TAKESHI;AIZAWA SHINICHI;IGAWA YOJI |
分类号 |
C12N5/10;C12N15/09;C12N15/31;(IPC1-7):C12N5/10 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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