USE OF TAQ POLYMERASE MUTANT ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS

摘要

The present invention generally relates to gene detection in standard and real-time PCR. One aspect of the invention provides mutant DNA polymerase e nzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. A nother aspect of the invention provides for methods of real-time polymerase chain reaction (PCR) assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another asp ect of the invention provides for methods of standard PCR assays using mutan t DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.