PSEUDOTYPED RETROVIRAL VECTORS AND METHODS OF MAKING AND USING THEM

摘要

Novel recombinant pseudotyped lentiviral or retroviral vectors comprising a nucleic acid encoding hemagglutinin, a nucleic acidneuraminidase and a nucleic acid a protease capable of cleaving a plurality of HA subtypes to their fusion-active form are useful in a variety of methods including methods for determining the effectiveness of vaccine-mediated immune response, methods for detecting the potency of neutralizing monoclonal antibodies, methods for detecting a compound that affects HA-mediated viral entry into a cell, methods for determining receptor specificity, methods for detecting an influenza infection. In addition, recombinant pseudotyped lentiviral or retroviral vectors comprising a nucleic acid encoding hemagglutinin without a nucleic acid encoding a protease and associated methods are also contemplated.